Dr Carolina Verissimo

BSc, Msc, PhD

 
researcher
 

Biography

Dr. Carolina De Marco Verissimo graduated in Biomedical Pathology and obtained her Master's degree in Agricultural and Environmental Microbiology in Brazil. Carolina received a joint-PhD in Cellular and Molecular Biology from PUCRS, Brazil and Griffith University, Australia. Her research focused on the characterization of glycans from helminth parasites to improve the diagnosis of angistrongyliasis and schistosimiasis infections. Carolina joined the Molecular Parasitology Laboratory in 2017, then at Queen's University Belfast. Her current work at NUI Galway focuses on the study of parasite (helminth) proteins and glycans involved in processes of infection, host immune regulation and pathology.

http://www.nuigalway.ie/science-engineering/school-of-natural-sciences/disciplines/zoology/research/...

@MPLNUIG on Twitter

Research Interests

I am interested on understanding how those parasites of great importance to human and animal health, agriculture and economy manipulate their host through the presentation and secretion-excretion of specific molecules (proteins and glycans), and with this to develop novel and effective alternatives to prevent parasitic infections. 

Research Projects

  Project Start Date End Date
Mining the molecular interplay between Parasite and Host for Vaccines, Diagnostics, and Biotherapeutics Discovery 01-FEB-19
Rapid ELISA test for SARS-CoV-2 (coronavirus) disease detection and survillence 15-MAY-20 02-MAY-21
Evaluation of Helmintex, a very sensitive Schistosoma mansoni eggs detection method & DNA 'barcoding' across Brazil and Africa isolates 03-APR-20

Book Chapters

  Year Publication
(2022) 'Applying `Omics¿ Technologies to Understand Fasciola spp. Biology'
Krystyna Cwiklinski, Carolina De Marco Verissimo, Paul McVeigh, Sheila Donnelly and John P. Dalton (2022) 'Applying `Omics¿ Technologies to Understand Fasciola spp. Biology' In: Fasciolosis 2nd Ed. :338-378 UK: CAB International. [DOI] [Details]
(2018) 'Helmintos'
Alessandra L. Morasssutti and Carolina De Marco Verissimo. (2018) 'Helmintos' In: Tratado de Analises Clinicas. 1ed. :493-508 Brazil: Editora Atheneu Ltda. [Details]

Peer Reviewed Journals

  Year Publication
(2022) 'Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins'
De Marco Verissimo C, Jewhurst HL, Dobó J, Gál P, Dalton JP, Cwiklinski K. (2022) 'Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins'. Plos Pathogens, 18 (1) [DOI] [Details]
(2022) 'Targeting Secreted Protease/Anti-Protease Balance as a Vaccine Strategy against the Helminth Fasciola hepatica'
Cwiklinski K, Drysdale O, López Corrales J, Corripio-Miyar Y, De Marco Verissimo C, Jewhurst H, Smith D, Lalor R, McNeilly TN, Dalton JP. (2022) 'Targeting Secreted Protease/Anti-Protease Balance as a Vaccine Strategy against the Helminth Fasciola hepatica'. Vaccine, 10 (2) [DOI] [Details]
(2022) 'Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system'
De Marco Verissimo C, Lopez-Corrales J, Dorey AL, Cwiklinski K, Lalor R, Calvani NED, Jewhurst HL, Doyle S, Dalton JP. (2022) 'Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system'. Epidemiology & Infection, [DOI] [Details]
(2022) 'Tuaimenal A, a Meroterpene from the Irish Deep-Sea Soft Coral Duva florida'
Avalon NE, Nafie J, De Marco Verissimo C, Warrensford LC, Dietrick SG, Pittman AR, Young RM, Kearns FL, Smalley T, Binning JM, Dalton JP, Johnson MP, Woodcock HL, Allcock AL, Baker BJ. (2022) 'Tuaimenal A, a Meroterpene from the Irish Deep-Sea Soft Coral Duva florida'. Journal Of Natural Products, [DOI] [Details]
(2021) 'Improved diagnosis of SARS-CoV-2 by using nucleoprotein and spike protein fragment 2 in quantitative dual ELISA tests'
De Marco Verissimo C, O'Brien C, López Corrales J, Dorey A, Cwiklinski K, Lalor R, Doyle JM, Field S, Masterson C, Ribes Martinez E, Hughes G, Bergin C, Walshe K, McNicholas B, Laffey JG, Dalton JP, Kerr C, Doyle S. (2021) 'Improved diagnosis of SARS-CoV-2 by using nucleoprotein and spike protein fragment 2 in quantitative dual ELISA tests'. Epidemiology & Infection, [DOI] [Details]
(2021) 'The Zoonotic Helminth Parasite Fasciola hepatica: Virulence-Associated Cathepsin B and Cathepsin L Cysteine Peptidases Secreted by Infective Newly Excysted Juveniles (NEJ)'
Barbour T, Cwiklinski K, Lalor R, Dalton JP, De Marco Verissimo C. (2021) 'The Zoonotic Helminth Parasite Fasciola hepatica: Virulence-Associated Cathepsin B and Cathepsin L Cysteine Peptidases Secreted by Infective Newly Excysted Juveniles (NEJ)'. Animals : An Open Access Journal From Mdpi, 11 (12) [DOI] [Details]
(2021) 'Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)'
López Corrales J, Cwiklinski K, De Marco Verissimo C, Dorey A, Lalor R, Jewhurst H, McEvoy A, Diskin M, Duffy C, Cosby SL, Keane OM, Dalton JP. (2021) 'Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)'. Veterinary Parasitology, 298 [DOI] [Details]
(2021) 'Pathogenicity and virulence of the liver flukes Fasciola hepatica and Fasciola gigantica that cause the zoonosis Fasciolosis'
Lalor R, Cwiklinski K, Calvani NED, Dorey A, Hamon S, Corrales JL, Dalton JP, De Marco Verissimo C. (2021) 'Pathogenicity and virulence of the liver flukes Fasciola hepatica and Fasciola gigantica that cause the zoonosis Fasciolosis'. Virulence, 12 (1):2839-2867 [DOI] [Details]
(2021) 'Autonomous Non Antioxidant Roles for Fasciola hepatica Secreted Thioredoxin-1 and Peroxiredoxin-1'
Dorey A, Cwiklinski K, Rooney J, De Marco Verissimo C, López Corrales J, Jewhurst H, Fazekas B, Calvani NED, Hamon S, Gaughan S, Dalton JP, Lalor R. (2021) 'Autonomous Non Antioxidant Roles for Fasciola hepatica Secreted Thioredoxin-1 and Peroxiredoxin-1'. Frontiers In Cellular And Infection Microbiology, 3 (11) [DOI] [Details]
(2020) 'Adaption of microbial communities to the hostile environment in the Doce River after the collapse of two iron ore tailing dams'
Giongo A;Dos Anjos Borges LG;Marconatto L;de Lara Palhano P;Serbent MP;Moreira-Silva E;de Abreu Siqueira T;Martinho CT;Barili R;Paz LV;Moser LI;De Marco Veríssimo C;Ketzer JMM;Medina-Silva R; (2020) 'Adaption of microbial communities to the hostile environment in the Doce River after the collapse of two iron ore tailing dams'. Heliyon, 6 (8) [DOI] [Details]
(2020) 'Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed 'clamp-like' mechanism of binding and inhibition'
Pritsch IC;Tikhonova IG;Jewhurst HL;Drysdale O;Cwiklinski K;Molento MB;Dalton JP;Verissimo CM; (2020) 'Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed 'clamp-like' mechanism of binding and inhibition'. Bmc Molecular And Cell Biology, 21 (1) [DOI] [Details]
(2020) 'Schistosomiasis: an epidemiological update on Brazil's southernmost low endemic area in Esteio'
Ramírez ADP;Favero V;Lindholz CG;Veríssimo CM;Pascoal VF;Candido RRF;Morassutti AL;Graeff-Teixeira C; (2020) 'Schistosomiasis: an epidemiological update on Brazil's southernmost low endemic area in Esteio'. Revista da Sociedade Brasileira de Medicina Tropical, 53 [DOI] [Details]
(2020) 'Fasciola hepaticaSerine Protease Inhibitor Family (Serpins): Purposely crafted for regulating host proteases'
Verissimo, CD;Jewhurst, HL;Tikhonova, IG;Urbanus, RT;Maule, AG;Dalton, JP;Cwiklinski, K (2020) 'Fasciola hepaticaSerine Protease Inhibitor Family (Serpins): Purposely crafted for regulating host proteases'. Plos Neglected Tropical Diseases, 14 [DOI] [Details]
(2020) 'A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method'
Favero, V;Verissimo, CD;Piovesan, AR;Morassutti, AL;Souto, AA;Bittencourt, HR;Pascoal, VF;Lindholz, CG;Jones, MK;Souza, RP;Rigo, FD;Carlini, CR;Graeff-Teixeira, C (2020) 'A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method'. Plos Neglected Tropical Diseases, 14 [DOI] [Details]
(2019) 'The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola'
Cwiklinski, K;Donnelly, S;Drysdale, O;Jewhurst, H;Smith, D;Verissimo, CD;Pritsch, IC;O'Neill, S;Dalton, JP;Robinson, MW (2019) 'The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola'. Advances In Parasitology, Vol 104, 104 :113-164 [DOI] [Details]
(2019) 'Glycans in the roles of parasitological diagnosis and host-parasite interplay'
Veríssimo CM;Graeff-Teixeira C;Jones MK;Morassutti AL; (2019) 'Glycans in the roles of parasitological diagnosis and host-parasite interplay'. Journal Of Parasitology, 146 (10) [DOI] [Details]
(2019) 'Qualitative and quantitative proteomic analyses of Schistosoma japonicum eggs and egg-derived secretory-excretory proteins'
De Marco Verissimo C;Potriquet J;You H;McManus DP;Mulvenna J;Jones MK; (2019) 'Qualitative and quantitative proteomic analyses of Schistosoma japonicum eggs and egg-derived secretory-excretory proteins'. Parasites & vectors, 12 (1) [DOI] [Details]
(2018) 'Study of diagnostic accuracy of Helmintex, Kato-Katz, and POC-CCA methods for diagnosing intestinal schistosomiasis in Candeal, a low intensity transmission area in northeastern Brazil'
Lindholz CG;Favero V;Verissimo CM;Candido RRF;de Souza RP;Dos Santos RR;Morassutti AL;Bittencourt HR;Jones MK;St Pierre TG;Graeff-Teixeira C; (2018) 'Study of diagnostic accuracy of Helmintex, Kato-Katz, and POC-CCA methods for diagnosing intestinal schistosomiasis in Candeal, a low intensity transmission area in northeastern Brazil'. Plos Neglected Tropical Diseases, 12 (3) [DOI] [Details]
(2017) 'Optimization of the Helmintex method for schistosomiasis diagnosis'
Favero V;Frasca Candido RR;De Marco Verissimo C;Jones MK;St Pierre TG;Lindholz CG;Da Silva VD;Morassutti AL;Graeff-Teixeira C; (2017) 'Optimization of the Helmintex method for schistosomiasis diagnosis'. Experimental Parasitology, 177 [DOI] [Details]
(2016) 'Comparative pathogenesis of eosinophilic meningitis caused by Angiostrongylus mackerrasae and Angiostrongylus cantonensis in murine and guinea pig models of human infection'
Aghazadeh M, Harvie MC, Owen HC, De Marco Veríssimo C, Aland KV, Reid SA, Traub RJ, McManus DP, McCarthy JS, Jones MK. (2016) 'Comparative pathogenesis of eosinophilic meningitis caused by Angiostrongylus mackerrasae and Angiostrongylus cantonensis in murine and guinea pig models of human infection'. Parasitology, 143 (10):1243-1251 [DOI] [Details]
(2016) 'Characterization of the N-glycans of female Angiostrongylus cantonensis worms'
Veríssimo CM;Morassutti AL;von Itzstein M;Sutov G;Hartley-Tassell L;McAtamney S;Dell A;Haslam SM;Graeff-Teixeira C; (2016) 'Characterization of the N-glycans of female Angiostrongylus cantonensis worms'. Experimental Parasitology, 166 [DOI] [Details]
(2013) 'Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp'
Veríssimo Cde M;Maschio VJ;Correa AP;Brandelli A;Rott MB; (2013) 'Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp'. Memorias do Instituto Oswaldo Cruz, 108 (7) [DOI] [Details]

Honours and Awards

  Year Title Awarding Body
2016 Endeavour Research Fellowship Australian Government
2014 International Scholarship for PhD Internship Coordination for the Improvement of Higher Education Personnel National Council for Scientific (CAPES), Brazil
2012 PhD Scholarship Pontifical Catholic University of Rio Grande do Sul (PUCRS), Brazil
2011 International Scholarship for MSc Internship National Council for Scientific and Technological Development (CNPq), Brazil
2010 MSc Scholarship National Council for Scientific and Technological Development (CNPq), Brazil
2006 BSc Scholarship Award ¿ Brazilian Program for University Access (ProUni), Brazil

Professional Associations

  Association Function From / To
International Staff Network, NUI Galway Member 01-JUN-20 /
Irish Society for Parasitology Member 01-JUN-18 /
British Society for Parasitology Member 01-SEP-18 /
Brazilian Society of Tropical Medicine Member 05-JUN-16 /

Employment

  Employer Position From / To
National University of Ireland Galway, Ireland Postdoctoral Researcher 06-JUN-19 /
Queen's University Belfast Postdoctoral fellow 02-APR-17 / 05-JUN-19
The University of Queensland Postdoctoral Fellow 01-FEB-16 / 08-JAN-17
Pontifical Catholic University of Rio Grande do Sul (PUCRS), Brazil. Postdoctoral Researcher 03-SEP-15 / 08-JAN-16
Hospital Irmandade da Santa Casa de Misericórdia, Brazil Biomedical Scientist 04-MAR-13 / 01-AUG-14
Fundação de Ciência e Tecnologia ¿ CIENTEC, Rio Grande do Sul, Brazil Biomedical Scientist 01-NOV-12 / 01-APR-13
Nobel and Bacellar Clinical Laboratory, Esteio Hospital ¿ Brazil Biomedical Scientist 06-FEB-11 / 05-APR-13

Education

  Year Institution Qualification Subject
2015 Pontifical Catholic University of Rio Grande do Sul (PUCRS) ¿ Brazil PhD in Molecular and Cellular Biology Medical and Molecular Parasitology
2015 Griffith University, Gold Coast, Australia PhD Research exchange Glycobiology
2012 Federal University of Rio Grande do Sul (UFRGS), Brazil MSc in Agricultural and Environmental Microbiology Characterisation of the proteolytic activity of environmental isolates and standard strains of Acanthamoeba spp. and its relationship with the in vivo pathogenicity
2011 National University of Rosario, Rosario, Argentina MSc research exchange Enzymology and applicability to Industry
2010 Centro Universitário Metodista, IPA-RS, Brazil BSc in Biomedicine Clinical-Pathology and Food Analyses

Languages

  Language
Portuguese
English
Spanish
Italian

Community Engagement

  Title Type From / To
Research (e.g. Community based research) Public engagement & Teaching in Parasitology 02-MAR-10 / 01-FEB-16

Reviews

  Journal Role
Scientific Reports Reviewer
International Journal For Parasitology Reviewer
Peerj Reviewer
Plos Neglected Tropical Diseases Reviewer
Animals : An Open Access Journal From Mdpi Reviewer

Recent Postgraduate Students

  Graduation Name Degree Primary Supervisor
2021 Muminat Kuye MSc in Biomedical Science
2021 Helena Bonar HONOURS BACHELOR DEGREE (LEVEL 8)
2021 Laoise Kelly HONOURS BACHELOR DEGREE (LEVEL 8)
2019 Izanara Pritsch PhD
2019 Orla Drysdale PhD
2019 James Rooney Msc

Modules Taught

  Term/Year Module Title Module Code Subject / Desc
2022/23 Biotechnology Module Biotechnology Module
2021 Inaugural module. Sweet Parasitology: How glycans contribute for parasitic infection. NA BSc in Biomedical Sciences and Biological Sciences, UniRitter - Brazil
2015 and 2020 Seminar series. Glycomics NA MSc and PhD students of the Molecular and Cellular Biology Program at PUCRS, Brazil
2013 and 2014 Winter Training Course. Theoretical and practical demonstrations in molecular tools for protein and glycan analysis. NA Undergraduate, MSc and PhD students at PUCRS, Brazil
2012 General and Clinical Parasitology NA BSc in Pharmacy, Biology and Biomedicine

Internal Collaborators

  Name Description of Collaboration
John P. Dalton

External Collaborators

  Name Organisation / Institute Country Description of Collaboration
Prof Malcolm Jones The University of Queensland AUSTRALIA
Prof Carlos Graeff-Teixeira Federal University of Sergipe, Brazil BRAZIL
Prof Patrick Skelly Tufts University U.S.A.
Dr Ekaterina Mirgorodskaya University of Gothenburg SWEDEN
Prof Sean Doyle Maynooth University IRELAND
Dr Niclas Karlsson University of Gothenburg SWEDEN